Human Gastric Adenocarcinoma Cell Line Essay -- Chemistry, Cell

IntroductionThere argon more different booth lines that have been used in fiat to examine genotoxicity exposure of agents such as gay gastric adenocarcinoma cellular phone line - TMK1, human colon cancer - COL2, particularly human B lymphoblastoid cell line - TK6 is more often used. TK6 is a cell line heterozygous at the thymidine kinase locus from a tolerant with chronic myelogenous leukemia (CML) in T cell lineage blast crisis which include Philadelphia chromosome t(922)(q34q11), an abnormality in chromosome 6 (a deletion of the long arm), and chromosome 7, for example, del(6)(q21) ins(1-)(q21-) del(1)(q21q32) dic(7)(p13 cen q3211.2 cen pter) (Watanabe et al., 1995 Tomita et al., 1998). These cells ar comparatively stable p53-normal immortal, have low mutation frequencies in gene and chromosome, and are proficient in mismatch repair (Tomita-Mitchel et al., 2000), from these properties TK6 cell line is apply in this study for assessing toxicity of anti-cancer agents. Polyyn es containing triple hold fasts which are presented in many natural sources, have been considered in medicine with a variety of biological activities such as antifungal, anti-inflammatory, antibacterial, antimicrobial, antiHIV, and anticancer (Dembitsky et al., 2006). Previously, there were many researches about the diyne-structure-related-capability of polyyne, particularly ene-diyne, these investigations suggested that enediyne compounds include two triple bonds (diyne) seperated by a double bond (ene) can cleavage DNA via cross linking to several positions, interacting with minor vallecula or abstracting hydrogen atoms as well as arrest cell cycle, inhibit proteins required for initiation replication stage (Chin et al., 1988 Sugiura et al., 1989 Walker et a... ... nuclei, and should be separated with clear nuclear boundary. Timescale involvedAlong with the proposal, the RAGS system go out be completed and approved in week 18. After that, the offset step is synthesis of two chemicals in 4 weeks from 16th January to thirteenth February 2012. Once the chemicals are produced, TK6 cells will be treated in direct medium and in medium containing these compounds, separately, and measure LD50 of TK6 cell line, statistic analysis LOEC, NOEC and thereby comparing cytotoxicity of these compounds. This process will take around 6 weeks from thirteenth February to 23rd March 2012. Later on, micronucleus assay will be use to study genotoxicity on TK6 cells via clastogenic behaviour for 7 weeks and finish laboratory possible on 1st June 2012. Finally, writing up and project draft will be completed within 4 weeks.